P-006 MACS Vs Microfluidics Sperm Sorting FOR RAISED SPERM DNA FRAGMENTATION INDEX – A RCT

Abstract Study question In Individuals with raised Sperm DNA Fragmentation Index (DFI), will sperm selection by magnetic activated cell sorting (MACS) or Microfluidic Sorting (MF) optimize the reproductive outcomes? Summary answer MACS MF offer comparable outcomes. There seems no superiority between them. spermDFI, MACS/MF can be considered to results. What is known already It evident that DFI negatively affects Management for outcomes still elusive. design, size, duration This an ongoing Randomized Control Trial (RCT) prior approval from institutional Ethical Committee (IEC) (ECR/1312/Inst/TG/2019). preliminary data of this study. 6months(July 2020–January 2021). Couples undergoing IVF stimulation spermDFI were randomized into MACS(n = 75) and MF(n 58) selection. study also intends look at efficiency reduce post intervention. trial registered Clinical Trials Registry India (CTRI) REF/2021/05/043439 Participants/materials, setting, methods one failed offered DFI. DFI>30% recruited. testing was done SCSA method using software. processing based on randomization day Oocyte Retrieval(OPU). as per manufacturer’s instructions. Culture till blastocyst stage freeze all policy followed. Two Blastocysts showed100% survival transferred in a Frozen Embryo transfer (FET) cycle. Main results role chance Implantation rates(IR) Miscarriage Rates (MR), Multiple Pregnancy Rates(MPR) Live Birth rates (LBR) compared groups Reduction value evaluated arm - 75 subjects recruited, 49 patients (65%) had atleast 2 blastocysts frozen underwent FET. 26 (35%) cancelled cycles due formation. 58 recruited 38 20 reduction Vs 83% men reduced < 30% intervention 87% Both seem efficient separate sperms good quality Reproductive Outcomes IR 50% 48% (p 0.8540) MR 5.3% 11% 0.3280) MPR 8% 18% 0.1624) LBR ET cycle 63% 1.0000) comparable. Data doesn’t prove over another Limitations, reasons caution presented here small sample size Wider implications findings Optimal management needs further research. However, both are non invasive Nevertheless while offering these aspects like cost, time taken process technical challenges if any, should appropriate counselling. registration number